Cytokeratin 8/18 Antibody
| Code | Size | Price |
|---|
| NSJ-V3316-0.1ML | 0.1 ml | £264.00 |
Quantity:
| NSJ-V3316-0.5ML | 0.5 ml | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 5D3
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Cytokeratin 8/18 antibody at 2-8oC.
Documents
Further Information
Application Details :
Flow cytometry: 2-5ul/million cells,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 1:50-1:100 for 30 min at RT
Application Note:
Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.
Description:
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
Format :
Bioreactor concentrate
Formulation :
Bioreactor concentrate with 0.05% sodium azide
Immunogen:
A cytoskeleton preparation from human breast cancer MCF-7 cells was used as the immunogen for the Cytokeratin 8/18 antibody.
Limitation:
This Cytokeratin 8/18 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Unpurified high titer supernatant
Species Reactivity :
Human
Uniprot #:
P05787, P05783