RXRA Antibody / Retinoid X Receptor alpha

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NSJ Bioreagents
Product Code: NSJ-RQ5942
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5942-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 5E7.
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Retinoid X Receptor alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human A549 cells with Retinoid X Receptor alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Retinoid X Receptor alpha antibody.
2 / 3
Flow cytometry testing of human Caco-2 cells with Retinoid X Receptor alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Retinoid X Receptor alpha antibody.
3 / 3
Western blot testing of human 1) HeLa, 2) A549, 3) HepG2, 4) Caco-2, 5) HL-60, 6) rat liver and 7) mouse liver lysate with Retinoid X Receptor alpha antibody. Predicted molecular weight: ~51 kDa but routinely observed at 54~60 kDa.
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Flow cytometry testing of human A549 cells with Retinoid X Receptor alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Retinoid X Receptor alpha antibody.
Flow cytometry testing of human Caco-2 cells with Retinoid X Receptor alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Retinoid X Receptor alpha antibody.
Western blot testing of human 1) HeLa, 2) A549, 3) HepG2, 4) Caco-2, 5) HL-60, 6) rat liver and 7) mouse liver lysate with Retinoid X Receptor alpha antibody. Predicted molecular weight: ~51 kDa but routinely observed at 54~60 kDa.

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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Retinoid X Receptor alpha antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Retinoid X receptor alpha (RXR-alpha), also known as NR2B1 (nuclear receptor subfamily 2, group B, member 1) is a nuclear receptor that in humans is encoded by the RXRA gene. Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A226-T462) was used as the immunogen for the Retinoid X Receptor alpha antibody.
Limitation:
This Retinoid X Receptor alpha antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P19793