APPL1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6235
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6235-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 5G11
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the APPL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human appendicitis tissue with APPL1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human breast cancer with APPL1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human rectal cancer with APPL1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) HL60, 2) ThP-1, 3) PC-3, 4) HeLa, 5) rat brain and 6) mouse brain lysate with APPL1 antibody. Predicted molecular weight ~80 kDa.
5 / 5
Flow cytometry testing of human U937 cells with APPL1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= APPL1 antibody.
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IHC staining of FFPE human appendicitis tissue with APPL1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with APPL1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with APPL1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HL60, 2) ThP-1, 3) PC-3, 4) HeLa, 5) rat brain and 6) mouse brain lysate with APPL1 antibody. Predicted molecular weight ~80 kDa.
Flow cytometry testing of human U937 cells with APPL1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= APPL1 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the APPL1 antibody should be determined by the researcher.
Description:
DCC-interacting protein 13-alpha (APPL1) is a protein that in humans is encoded by the APPL1 gene. The APPL1 gene is mapped to 3q21.1-p13.3. It is said to contain 709 amino acids and share 54% amino acid identity with APPL2. APPL is highly expressed in skeletal muscle, heart, ovary, and pancreas, tissues in which AKT2 mRNA is abundant. It has been regarded as an adaptor that may tether inactive AKT2 to the PI3K in the cytoplasm and thereby may expedite recruitment of AKT2 and PI3K to the cell membrane upon mitogenic stimulation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids K91-R668) was used as the immunogen for the APPL1 antibody.
Limitation:
This APPL1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UKG1