MPI Antibody / Mannose Phosphate Isomerase

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NSJ Bioreagents
Product Code: NSJ-RQ6274
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6274-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 5G5
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Mannose Phosphate Isomerase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 7
Immunofluorescent staining of FFPE human T-47D cells with Mannose Phosphate Isomerase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
IHC staining of FFPE human ovarian serous adenocarcinoma with Mannose Phosphate Isomerase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human lung cancer with Mannose Phosphate Isomerase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human breast cancer with Mannose Phosphate Isomerase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human renal clear cell carcinoma with (right) and without (left) Mannose Phosphate Isomerase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
Western blot testing of human 1) HeLa, 2) Caco-2, 3) HEK293, 4) U-87 MG and 5) rat brain lysate with Mannose Phosphate Isomerase antibody. Predicted molecular weight ~47 kDa.
7 / 7
Flow cytometry testing of human U-87 MG cells with Mannose Phosphate Isomerase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Mannose Phosphate Isomerase antibody.
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Immunofluorescent staining of FFPE human T-47D cells with Mannose Phosphate Isomerase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human ovarian serous adenocarcinoma with Mannose Phosphate Isomerase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with Mannose Phosphate Isomerase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with Mannose Phosphate Isomerase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal clear cell carcinoma with (right) and without (left) Mannose Phosphate Isomerase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) Caco-2, 3) HEK293, 4) U-87 MG and 5) rat brain lysate with Mannose Phosphate Isomerase antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human U-87 MG cells with Mannose Phosphate Isomerase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Mannose Phosphate Isomerase antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Mannose Phosphate Isomerase antibody should be determined by the researcher.
Description:
Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate(F6P) and mannose-6-phosphate(M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids A2-K99) was used as the immunogen for the Mannose Phosphate Isomerase antibody.
Limitation:
This Mannose Phosphate Isomerase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P34949