Beta Catenin Antibody

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NSJ Bioreagents
Product Code: NSJ-V3249
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3249-20UG20 ug£264.00
Quantity:
NSJ-V3249-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 5H10
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Beta Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human tonsil tissue with Beta Catenin antibody (clone 5H10). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.~
2 / 2
Western blot testing of human 1) A431 and 2) A549 cell lysate with B
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IHC testing of FFPE human tonsil tissue with Beta Catenin antibody (clone 5H10). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.~
Western blot testing of human 1) A431 and 2) A549 cell lysate with B

Documents

Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Beta Catenin antibody to be determined by the researcher.
Description:
Beta Catenin is a dual function protein, regulating the coordination of cell?cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. Beta catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-Catenin, also known as plakoglobin. Beta-Catenin is widely expressed in many tissues. In cardiac muscle, it localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocyte. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A C-terminal 100 amino acid segment from chicken beta-Catenin was used as the immunogen for the Beta Catenin antibody.
Limitation:
This Beta Catenin antibody is available for research use only.
Localization:
Cell surface, cytoplasmic, cell junctions
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse
Uniprot #:
P35222