ATP Citrate Lyase Antibody
Code | Size | Price |
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NSJ-RQ5221-100ug | 100 ug | £535.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 5I2
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
After reconstitution, the ATP Citrate Lyase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Further Information
Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry(FFPE): 0.5-1ug/ml,Flow Cytometry: 1-3ug/1x10^6 cells,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the ATP Citrate Lyase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
ATP citrate lyase, aslo known as ACLY,is anenzymethat in animals represents an important step infatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis ofcytosolicacetyl-CoAin many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, includinglipogenesis andcholesterogenesis.It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids M1-I180) was used as the immunogen for the ATP Citrate Lyase antibody.
Limitation:
This ATP Citrate Lyase antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P53396