BUB1B Antibody / BubR1

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NSJ Bioreagents
Product Code: NSJ-RQ5672
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5672-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 5I7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the BUB1B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human breast cancer with BUB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer with BUB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human ovarian cancer with BUB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer with BUB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human A431 cells with BUB1B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human HeLa cells with BUB1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BUB1B antibody.
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Western blot testing of human 1) HeLa, 2) HEK293 and 3) K562 lysate with BUB1B antibody. Predicted molecular weight ~120 kDa.
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IHC staining of FFPE human breast cancer with BUB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with BUB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with BUB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with BUB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with BUB1B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HeLa cells with BUB1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BUB1B antibody.
Western blot testing of human 1) HeLa, 2) HEK293 and 3) K562 lysate with BUB1B antibody. Predicted molecular weight ~120 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the BUB1B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta is an enzyme that in humans is encoded by the BUB1B gene. This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K26-E448) was used as the immunogen for the BUB1B antibody.
Limitation:
This BUB1B antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
O60566