Transferrin Receptor Antibody / TFRC / CD71

NSJ Bioreagents
Product Code: NSJ-V2261
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2261-20UG20 ug£264.00
Quantity:
NSJ-V2261-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 66IG10
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Store the Transferrin receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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FACS testing of K562 cells with Alexa Fluor 488 conjugated Transferrin receptor antibody (green) and isotype control (gray).~
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SDS-PAGE Analysis of Purified, BSA-Free Transferrin Receptor Antibody (clone 66IG10). Confirmation of Integrity

FACS testing of K562 cells with Alexa Fluor 488 conjugated Transferrin receptor antibody (green) and isotype control (gray).~
SDS-PAGE Analysis of Purified, BSA-Free Transferrin Receptor Antibody (clone 66IG10). Confirmation of Integrity

Further Information

Application Details :
ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Transferrin receptor antibody to be titered up or down for optimal performance.
Description:
This antibody recognizes a ~85-95kDa protein which is identified as cell surface Transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. The antibody is highly specific to transferrin receptor and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of transferrin receptor. Its ligand is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
7037
Immunogen:
Recombinant human protein was used as the immunogen for this Transferrin receptor antibody.
Limitation:
This Transferrin receptor antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human