EGF Receptor Antibody

NSJ Bioreagents
Product Code: NSJ-F40405
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40405-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40405-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 688CT33.1.3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the EGF Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunohistochemical analysis of paraffin-embedded human esophagus using EGF Receptor antibody at 1:25 dilution.
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Immunohistochemical analysis of paraffin-embedded human stomach using EGF Receptor antibody at 1:25 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse esophagus using EGF Receptor antibody at 1:25 dilution.
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Immunohistochemical analysis of paraffin-embedded rat esophagus using EGF Receptor antibody at 1:25 dilution.
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Western blot analysis of lysate from A431, MCF-7 cell line (left to right) using EGF Receptor antibody at 1:1000 for each lane.
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EGF Receptor antibody western blot analysis in HeLa lysate

Immunohistochemical analysis of paraffin-embedded human esophagus using EGF Receptor antibody at 1:25 dilution.
Immunohistochemical analysis of paraffin-embedded human stomach using EGF Receptor antibody at 1:25 dilution.
Immunohistochemical analysis of paraffin-embedded mouse esophagus using EGF Receptor antibody at 1:25 dilution.
Immunohistochemical analysis of paraffin-embedded rat esophagus using EGF Receptor antibody at 1:25 dilution.
Western blot analysis of lysate from A431, MCF-7 cell line (left to right) using EGF Receptor antibody at 1:1000 for each lane.
EGF Receptor antibody western blot analysis in HeLa lysate

Further Information

Application Details :
IHC (Paraffin): 1:25,Western blot: 1:1000
Application Note:
Titration of the EGF Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1163-1191 from the human protein was used as the immunogen for this EGF Receptor antibody.
Limitation:
This EGF Receptor antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P00533