RAB11B Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5671
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5671-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 6C5
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the RAB11B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human MCF7 cells with RAB11B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Flow cytometry testing of human Caco-2 cells with RAB11B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB11B antibody.
3 / 4
Western blot testing of human 1) HEK293, 2) HeLa, 3) A549, 4) placenta, 5) HepG2, 6) Caco-2, 7) ThP-1 and 8) Raji lysate with RAB11B antibody. Predicted molecular weight ~24 kDa.
4 / 4
Western blot testing of rat 1) brain, 2) lung, 3) spleen, 4) C6 and mouse 5) brain, 6) lung, 7) spleen, 8) Neuro-2a and 9) RAW264.7 lysate with RAB11B antibody. Predicted molecular weight ~24 kDa.
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Immunofluorescent staining of FFPE human MCF7 cells with RAB11B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human Caco-2 cells with RAB11B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB11B antibody.
Western blot testing of human 1) HEK293, 2) HeLa, 3) A549, 4) placenta, 5) HepG2, 6) Caco-2, 7) ThP-1 and 8) Raji lysate with RAB11B antibody. Predicted molecular weight ~24 kDa.
Western blot testing of rat 1) brain, 2) lung, 3) spleen, 4) C6 and mouse 5) brain, 6) lung, 7) spleen, 8) Neuro-2a and 9) RAW264.7 lysate with RAB11B antibody. Predicted molecular weight ~24 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the RAB11B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Ras-related protein Rab-11B is a protein that in humans is encoded by the RAB11B gene. It is mapped to 19p13.2. The Ras superfamily of small GTP-binding proteins, which includes the Ras, Ral, Rho, Rap, and Rab families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QKQIADRAAHDESPGNNVVDISVPPTTDGQKPNKLQCC from the human protein were used as the immunogen for the RAB11B antibody.
Limitation:
This RAB11B antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15907