Clathrin heavy chain Antibody / CLTC

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NSJ Bioreagents
Product Code: NSJ-RQ6408
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6408-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 6D3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Clathrin heavy chain antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HepG2 cells with Clathrin heavy chain antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Clathrin heavy chain antibody.
2 / 5
Western blot testing of 1) human HeLa, 2) human A431, 3) rat brain and 4) mouse brain tissue lysate with Clathrin heavy chain antibody. Predicted molecular weight ~192 kDa.
3 / 5
IHC staining of FFPE human tonsil tissue with Clathrin heavy chain antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE human pancreatic cancer tissue with Clathrin heavy chain antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 5
IHC staining of FFPE human renal carcinoma tissue with Clathrin heavy chain antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Flow cytometry testing of human HepG2 cells with Clathrin heavy chain antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Clathrin heavy chain antibody.
Western blot testing of 1) human HeLa, 2) human A431, 3) rat brain and 4) mouse brain tissue lysate with Clathrin heavy chain antibody. Predicted molecular weight ~192 kDa.
IHC staining of FFPE human tonsil tissue with Clathrin heavy chain antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic cancer tissue with Clathrin heavy chain antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal carcinoma tissue with Clathrin heavy chain antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Clathrin heavy chain antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Clathrin heavy chain 1 is a protein that in humans is encoded by the CLTC gene. Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R967-Q1668) was used as the immunogen for the Clathrin heavy chain antibody.
Limitation:
This Clathrin heavy chain antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q00610