Peroxiredoxin 6 Antibody / PRDX6 / aiPLA2

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NSJ Bioreagents
Product Code: NSJ-RQ6026
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6026-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 6I8
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Peroxiredoxin 6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human A549 cells with Peroxiredoxin 6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HEK293, 4) K562, 5) SW620 and 6) mouse RH35 antibody with Peroxiredoxin 6 antibody. Expected molecular weight: ~25 kDa.
3 / 4
Flow cytometry testing of human HeLa cells with Peroxiredoxin 6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Peroxiredoxin 6 antibody.
4 / 4
IHC staining of FFPE human endometrial carcinoma cancer with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human A549 cells with Peroxiredoxin 6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HEK293, 4) K562, 5) SW620 and 6) mouse RH35 antibody with Peroxiredoxin 6 antibody. Expected molecular weight: ~25 kDa.
Flow cytometry testing of human HeLa cells with Peroxiredoxin 6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Peroxiredoxin 6 antibody.
IHC staining of FFPE human endometrial carcinoma cancer with Peroxiredoxin 6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Peroxiredoxin 6 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
PRDX6 is also known as PRX, p29 or AOP2. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E15-P224) was used as the immunogen for the Peroxiredoxin 6 antibody.
Limitation:
This Peroxiredoxin 6 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P30041