PAH Antibody / Phenylalanine hydroxylase

Contact us
NSJ Bioreagents
Product Code: NSJ-F54526
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54526-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54526-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 717CT21.1.1
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Phenylalanine hydroxylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human liver tissue with Phenylalanine hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human kidney tissue with Phenylalanine hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
IHC testing of FFPE mouse kidney tissue with Phenylalanine hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Western blot testing of 1) human HepG2, 2) mouse liver and 3) rat liver lysate with Phenylalanine hydroxylase antibody. Predicted molecular weight ~52 kDa.
❮❯

IHC testing of FFPE human liver tissue with Phenylalanine hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with Phenylalanine hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse kidney tissue with Phenylalanine hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human HepG2, 2) mouse liver and 3) rat liver lysate with Phenylalanine hydroxylase antibody. Predicted molecular weight ~52 kDa.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Phenylalanine hydroxylase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Phenylalanine hydroxylase / PAH catalyzes the hydroxylation of L-phenylalanine to L-tyrosine. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the Phenylalanine hydroxylase antibody.
Limitation:
This Phenylalanine hydroxylase antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P00439