TBP Antibody (TATA Binding Protein)

NSJ Bioreagents
Product Code: NSJ-F52225
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52225-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52225-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 830CT4.3.3
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TBP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of 1) human 293, 2) (h) HeLa, 3) (h) MDA-MB-231, 4) rat H-4-II-E, 5) (h) HCT-116 and 6) mouse C2C12 cell lysate with TBP antibody at 1:2000. Expected molecular weight: 35-43 kDa.
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TBP antibody western blot analysis in (1) human HeLa, (2) (h) HepG2, (3) mouse NIH3T3 lysate. Expected molecular weight: 35-43 kDa.
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Immunohistochemical analysis of paraffin-embedded human testis using TBP antibody at 1:25 dilution.
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IHC testing of FFPE human testis tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.
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IHC testing of FFPE mouse brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.
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IHC testing of FFPE rat brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.
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IHC testing of FFPE monkey brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.

Western blot testing of 1) human 293, 2) (h) HeLa, 3) (h) MDA-MB-231, 4) rat H-4-II-E, 5) (h) HCT-116 and 6) mouse C2C12 cell lysate with TBP antibody at 1:2000. Expected molecular weight: 35-43 kDa.
TBP antibody western blot analysis in (1) human HeLa, (2) (h) HepG2, (3) mouse NIH3T3 lysate. Expected molecular weight: 35-43 kDa.
Immunohistochemical analysis of paraffin-embedded human testis using TBP antibody at 1:25 dilution.
IHC testing of FFPE human testis tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.
IHC testing of FFPE mouse brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.
IHC testing of FFPE rat brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.
IHC testing of FFPE monkey brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.

Further Information

Application Details :
IHC (Paraffin): 1:25,Western blot: 1:1000-2:000
Application Note:
Titration of the TBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Purified His-tagged protein was used to produced this monoclonal TBP antibody.
Limitation:
This TBP antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
P20226