CBX8 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6366
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6366-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 8G7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CBX8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human gallbladder adenocarcinoma tissue with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human 1) HeLa, 2) A549 and 3) Raji cell lysate with CBX8 antibody. Predicted molecular weight ~43 kDa.
3 / 3
Flow cytometry testing of human HL60 cells with CBX8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CBX8 antibody.
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IHC staining of FFPE human gallbladder adenocarcinoma tissue with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) A549 and 3) Raji cell lysate with CBX8 antibody. Predicted molecular weight ~43 kDa.
Flow cytometry testing of human HL60 cells with CBX8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CBX8 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the CBX8 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Chromobox protein homolog 8 functions as a transcriptional repressor and has a role in DNA damage response.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-R389) was used as the immunogen for the CBX8 antibody.
Limitation:
This CBX8 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9HC52