Beta Actin Antibody

NSJ Bioreagents
Product Code: NSJ-F40187
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40187-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40187-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG
Antibody Clonality: Monoclonal
Antibody Clone: 8H10D10
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the beta-Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot analysis of lysate from HepG2, HL-60 cell line (left to right) using beta-Actin antibody at 1:1000 for each lane.
2 / 4
Immunohistochemical analysis of paraffin-embedded human spleen using beta-Actin antibody at 1:25 dilution.
3 / 4
Western blot analysis of lysate from HepG2, HL-60, mouse NIH3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 lysate (left to right) using beta-Actin antibody at 1:1000 for each lane.
4 / 4
Beta-Actin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma.

Western blot analysis of lysate from HepG2, HL-60 cell line (left to right) using beta-Actin antibody at 1:1000 for each lane.
Immunohistochemical analysis of paraffin-embedded human spleen using beta-Actin antibody at 1:25 dilution.
Western blot analysis of lysate from HepG2, HL-60, mouse NIH3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 lysate (left to right) using beta-Actin antibody at 1:1000 for each lane.
Beta-Actin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the beta-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus. It is classically referred to as being a 'nonmuscle' actin, however expression is ubiquitous, strongly in lymph tissue and weakly in heart and muscle.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
ACTB recombinant protein was used to produce this monoclonal beta Actin antibody.
Limitation:
This beta-Actin antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P60709