Histone H2AX Antibody / H2AFX

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NSJ Bioreagents
Product Code: NSJ-F54477
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54477-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54477-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 938CT5.1.1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Histone H2AX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human thymus tissue with Histone H2AX antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human thymus tissue with Histone H2AX antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
IHC testing of FFPE human prostate tissue with Histone H2AX antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Western blot testing of human 1) HEK293, 2) CEM, 3) HepG2, 4) Jurkat, 5) HeLa and 6) Raji cell lysate with Histone H2AX antibody. Predicted molecular weight ~15 kDa.
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IHC testing of FFPE human thymus tissue with Histone H2AX antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human thymus tissue with Histone H2AX antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human prostate tissue with Histone H2AX antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HEK293, 2) CEM, 3) HepG2, 4) Jurkat, 5) HeLa and 6) Raji cell lysate with Histone H2AX antibody. Predicted molecular weight ~15 kDa.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 115-143 from the human protein was used as the immunogen for the Histone H2AX antibody.
Limitation:
This Histone H2AX antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P16104