CD10 Antibody / Neprilysin

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NSJ Bioreagents
Product Code: NSJ-F54497
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54497-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54497-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 965CT9.1.1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CD10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human kidney tissue with CD10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human kidney tissue with CD10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) placenta, 2) Ramos and 3) kidney lysate with CD10 antibody. Routinely visualized at ~100 kDa.
4 / 4
Flow cytometry testing of fixed human Jurkat cells with CD10 antibody; Blue=isotype control, Green= CD10 antibody.
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IHC testing of FFPE human kidney tissue with CD10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with CD10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) placenta, 2) Ramos and 3) kidney lysate with CD10 antibody. Routinely visualized at ~100 kDa.
Flow cytometry testing of fixed human Jurkat cells with CD10 antibody; Blue=isotype control, Green= CD10 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the CD10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the CD10 antibody.
Limitation:
This CD10 antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P08473