SUMO-2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F52344
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52344-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52344-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 973CT8.1.1
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the SUMO-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Fluorescent image of HeLa cells stained with SUMO-2 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).~
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Fluorescent image of HeLa cells stained with SUMO-2 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).~

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:1000
Application Note:
Titration of the SUMO-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Purified His-tagged protein was used to produced this monoclonal SUMO-2 antibody.
Limitation:
This SUMO-2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Rat
Species Reactivity (Predicted):
Bovine, Chicken, Hamster, Mouse, Pig, Primate
Uniprot #:
P61956