KRT19 Antibody / Cytokeratin 19

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NSJ Bioreagents
Product Code: NSJ-F54479
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54479-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54479-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1,k
Antibody Clonality: Monoclonal
Antibody Clone: 979CT14.3.1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Keratin 19 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human kidney tissue with Keratin 19 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human colon tissue with Keratin 19 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Keratin 19 antibody (green) and DAPI nuclear stain (blue).
4 / 4
Western blot testing of human 1) HepG2 and 2) MCF7 cell lysate with Keratin 19 antibody. Predicted molecular weight ~43 kDa.
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IHC testing of FFPE human kidney tissue with Keratin 19 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon tissue with Keratin 19 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Keratin 19 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human 1) HepG2 and 2) MCF7 cell lysate with Keratin 19 antibody. Predicted molecular weight ~43 kDa.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Keratin 19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the Keratin 19 antibody.
Limitation:
This Keratin 19 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P08727