GST Tag Antibody

NSJ Bioreagents
Product Code: NSJ-F52060
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52060-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52060-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 9AT106
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot the GST Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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GST Tag antibody used in western blot with recombinant protein~
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Western blot analysis of lysate from 12 tag recombinant protein (41 kDa) using GST Tag antibody at 1:1000.

GST Tag antibody used in western blot with recombinant protein~
Western blot analysis of lysate from 12 tag recombinant protein (41 kDa) using GST Tag antibody at 1:1000.

Further Information

Application Details :
Western blot: 1:1000-2000
Application Note:
Titration of the GST Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Purified recombinant fusion protein was used to produced this monoclonal GST tag antibody.
Limitation:
This GST Tag antibody is available for research use only.
Purity:
Purified