TRIM28 Antibody / KAP1

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NSJ Bioreagents
Product Code: NSJ-RQ5933
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5933-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 9E3.
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TRIM28 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human breast cancer with TRIM28 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer with TRIM28 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat intestine with TRIM28 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of frozen human placenta with TRIM28 antibody.
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Immunofluorescent staining of FFPE human rectal cancer with TRIM28 antibody (green). HIER: steam section in pH8 EDTA for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with TRIM28 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human 1) HeLa, 2) PC-3, 3) HEK293, 4) A549, 5) Jurkat, 6) ThP-1, 7) rat PC-12 and 8) mouse NIH 3T3 lysate with TRIM28 antibody. Observed molecular weight: 88~110 kDa depending on sumoylation.
8 / 8
Flow cytometry testing of human A549 cells with TRIM28 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRIM28 antibody.
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IHC staining of FFPE human breast cancer with TRIM28 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with TRIM28 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with TRIM28 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen human placenta with TRIM28 antibody.
Immunofluorescent staining of FFPE human rectal cancer with TRIM28 antibody (green). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with TRIM28 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) PC-3, 3) HEK293, 4) A549, 5) Jurkat, 6) ThP-1, 7) rat PC-12 and 8) mouse NIH 3T3 lysate with TRIM28 antibody. Observed molecular weight: 88~110 kDa depending on sumoylation.
Flow cytometry testing of human A549 cells with TRIM28 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRIM28 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE and Frozen): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the TRIM28 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1 beta (TIF1b) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A699-P835) was used as the immunogen for the TRIM28 antibody.
Limitation:
This TRIM28 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13263