Blood Group Lewis y Antibody

NSJ Bioreagents
Product Code: NSJ-V3309
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3309-20UG20 ug£264.00
Quantity:
NSJ-V3309-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: A70-A/A9
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Lewis y antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human colon carcinoma with Lewis y antibody (clone A70-A/A9). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.~
2 / 2
SDS-PAGE analysis of purified, BSA-free Lewis y antibody (clone A70-A/A9)

IHC testing of FFPE human colon carcinoma with Lewis y antibody (clone A70-A/A9). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.~
SDS-PAGE analysis of purified, BSA-free Lewis y antibody (clone A70-A/A9)

Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Lewis y antibody should be determined by the researcher.
Description:
This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Live Ls174T cells (human colon carcinoma) were used as the immunogen for the Lewis y antibody.
Limitation:
This Lewis y antibody is available for research use only.
Localization:
Cell membrane, cytoplasmic and extracellular
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Not Applicable