ATF6 Antibody

Western blot: 2-4ug/ml (2),IHC (Paraffin): 5ug/ml (1)

ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, pH 7.4

ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.

Purified

0.5 mg/ml antibody in PBS with 0.1 mg/ml BSA (US source) and 0.05% sodium azide

22926

A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.

This ATF6 antibody is available for research use only.

Cytoplasmic

Protein G purified monoclonal antibody

Human, Mouse, Rat