AKT1 Antibody
| Code | Size | Price |
|---|
| NSJ-V8442-20UG | 20 ug | £264.00 |
Quantity:
| NSJ-V8442-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: AKT1/3898R
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details :
Western blot: 1-2ug/ml,ELISA: order Ab without BSA for coating
Application Note:
Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.
Description:
Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 85-189 from the human protein was used as the immunogen for the recombinant AKT1 antibody.
Limitation:
This recombinant AKT1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic, cell surface
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P31749