Bcl-2 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3671
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3671-20UG20 ug£264.00
Quantity:
NSJ-V3671-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: ARBC2-2R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human spleen tissue with recombinant Bcl-2 antibody (clone ARBC2-2R). HIER: boil tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.~
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Western blot testing of human MCF7
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IHC testing of FFPE human spleen tissue with recombinant Bcl-2 antibody (clone ARBC2-2R). HIER: boil tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.~
Western blot testing of human MCF7

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
The optimal dilution of the recombinant Bcl-2 antibody for each application should be determined by the researcher.
Description:
This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant full length human protein was used as the immunogen for this recombinant Bcl-2 antibody.
Limitation:
This recombinant Bcl-2 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P10415