Basic Cytokeratin Antibody

Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

Titering of the Basic Cytokeratin antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. This Basic Cytokeratin antibody detects the following keratins/CKs: CK1 (67kDa), CK3 (64kDa), CK4 (59kDa), CK5 (58 kDa), CK6 (56kDa), CK8 (52 kDa).

There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

An amino acid sequence common to basic keratins was used as the immunogen for the Basic Cytokeratin antibody.

This Basic Cytokeratin antibody is available for research use only.

Cytoplasmic

Protein G affinity chromatography

Human, Rat. Broad species reactivity expected.

Q01546