EpCAM Antibody

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NSJ Bioreagents
Product Code: NSJ-V7934
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7934-0.1ML0.1 ml£264.00
Quantity:
NSJ-V7934-0.5ML0.5 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: Ber-EP4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Flow cytometry testing of human MCF7 cells with EpCAM antibody (clone Ber-EP4); Red=isotype control, Blue= EpCAM antibody.
2 / 3
IHC staining of FFPE human prostate carcinoma with EpCAM antibody (clone Ber-EP4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 3
IHC staining of FFPE human breast carcinoma with EpCAM antibody (clone Ber-EP4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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Flow cytometry testing of human MCF7 cells with EpCAM antibody (clone Ber-EP4); Red=isotype control, Blue= EpCAM antibody.
IHC staining of FFPE human prostate carcinoma with EpCAM antibody (clone Ber-EP4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma with EpCAM antibody (clone Ber-EP4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:100-1:200,Flow cytometry: 1:100-1:200
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. It has been reported that this epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
Format :
Culture supernatant
Formulation :
Culture supernatant with 0.05% sodium azide
Immunogen:
Human breast cancer MCF-7 cells were used as the immunogen for the EpCAM antibody.
Limitation:
This EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Unpurified high titer supernatant
Species Reactivity :
Human
Uniprot #:
P16422