Biotin / Vitamin B7 / Vitamin H Antibody

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NSJ Bioreagents
Product Code: NSJ-V3649
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3649-20UG20 ug£264.00
Quantity:
NSJ-V3649-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: BTN/2032R
Regulatory Status: RUO
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC: Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Kappa Light Chain Ab probe followed by anti-Biotin antibody (clone BTN/2032R). No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.~
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IHC: Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Kappa Light Chain Ab probe followed by anti-Biotin antibody (clone BTN/2032R). No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.~

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The optimal dilution of the recombinant Biotin antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Biotinylated sheep immunoglobulin was used as the immunogen for this recombinant Biotin antibody.
Limitation:
This recombinant Biotin antibody is available for research use only.
Localization:
target location
Purity:
Protein A affinity chromatography
Species Reactivity :
Biotin
Uniprot #:
Not Applicable