Alpha Fetoprotein / AFP Antibody

NSJ Bioreagents
Product Code: NSJ-V2002
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2002-20UG20 ug£264.00
Quantity:
NSJ-V2002-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: C3
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the AFP antibody at 2-8°C.

Images

1 / 2
IHC testing of human fetal liver stained with AFP antibody (C3).~
2 / 2
SDS-PAGE Analysis of Purified, BSA-Free AFP Antibody (clone C3). Confirmation of Integrity and Purity of the Antibody.

IHC testing of human fetal liver stained with AFP antibody (C3).~
SDS-PAGE Analysis of Purified, BSA-Free AFP Antibody (clone C3). Confirmation of Integrity and Purity of the Antibody.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the AFP antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as Alpha fetoprotein (AFP). The AFP antibody is highly specific and shows no cross-reaction with other oncofetal antigens or serum albumin. Alpha fetoprotein is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. AFP antibody has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
174
Immunogen:
Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.
Limitation:
This Alpha Fetoprotein / AFP antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human