CD31 Antibody / PECAM-1

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NSJ Bioreagents
Product Code: NSJ-V7243
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7243-20UG20 ug£264.00
Quantity:
NSJ-V7243-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: C31/1395R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant CD31 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
2 / 3
IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant CD31 antibody (clone C31/1395R) as confirmation of integrity and purity.
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IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant CD31 antibody (clone C31/1395R) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Titering of the recombinant CD31 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
5175
Immunogen:
Human full length recombinant protein was used as the immunogen for the recombinant CD31 antibody.
Limitation:
This recombinant CD31 antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein A affinity
Species Reactivity :
Human
Uniprot #:
P16284