Cytokeratin 5/8 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3059
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3059-20UG20 ug£264.00
Quantity:
NSJ-V3059-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: C-50
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytokeratin 5/8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (clone C-50). Required HIER: digest sections with Trypsin at 1mg/ml, 15 min, at RT.~
2 / 2
Western blot testing of HeLa cell lysate a
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IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (clone C-50). Required HIER: digest sections with Trypsin at 1mg/ml, 15 min, at RT.~
Western blot testing of HeLa cell lysate a

Documents

Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.
Limitation:
This Cytokeratin 5/8 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P13647, P05787