Calbindin 2 Antibody / CALB2 / Calretinin

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NSJ Bioreagents
Product Code: NSJ-V8172
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8172-20UG20 ug£264.00
Quantity:
NSJ-V8172-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CALB2/2602
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CALB2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human testicular carcinoma with CALB2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2 / 6
IHC testing of FFPE human cerebellum with CALB2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 6
IHC testing of FFPE human mesothelioma with CALB2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
4 / 6
Western blot testing of human brain lysate with CALB2 antibody. Expected molecular weight ~29 kDa.
5 / 6
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CALB2 antibody (clone CALB2/2602). These results demonstrate the foremost specificity of the CALB2/2602 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
6 / 6
SDS-PAGE analysis of purified, BSA-free CALB2 antibody as confirmation of integrity and purity.
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IHC testing of FFPE human testicular carcinoma with CALB2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human cerebellum with CALB2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human mesothelioma with CALB2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human brain lysate with CALB2 antibody. Expected molecular weight ~29 kDa.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CALB2 antibody (clone CALB2/2602). These results demonstrate the foremost specificity of the CALB2/2602 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CALB2 antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2). Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 23-242 from the human protein was used as the immunogen for the Calretinin antibody.
Limitation:
This CALB2 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P22676