Calbindin 2 Antibody / CALB2 / Calretinin

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NSJ Bioreagents
Product Code: NSJ-V7366
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7366-20UG20 ug£264.00
Quantity:
NSJ-V7366-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CALB2/2685
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Calbindin 2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human Mesothelioma with Calbindin 2 antibody (clone CALB2/2685). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2 / 3
IHC testing of FFPE human cerebellum with Calbindin 2 antibody (clone CALB2/2685). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 3
SDS-PAGE analysis of purified, BSA-free Calbindin 2 antibody (clone CALB2/2685) as confirmation of integrity and purity.
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IHC testing of FFPE human Mesothelioma with Calbindin 2 antibody (clone CALB2/2685). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human cerebellum with Calbindin 2 antibody (clone CALB2/2685). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free Calbindin 2 antibody (clone CALB2/2685) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Calbindin 2 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2). Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 23-242 from the human protein was used as the immunogen for the Calbindin 2 antibody.
Limitation:
This Calbindin 2 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P22676