Caspase-3 Antibody, Pro and Active
| Code | Size | Price |
|---|
| NSJ-N1045-25UG | 25 ug | £254.00 |
Quantity:
| NSJ-N1045-100UG | 100 ug | £450.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CAS3836
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Aliquot the Pro and Active Caspase-3 antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details :
Western blot: 2-4ug/ml,IHC (FFPE): 5ug/ml
Application Note:
This antibody will detect the pro form and p17 and p12 active forms of Caspase-3.
Buffer:
1X PBS, pH 7.4
Description:
Caspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. They are often referred to as the 'executioners of apoptosis.' Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. Active caspase-1 and -3 are heterodimers, containing two small and two large subunits. Active Caspase-3 subunits are 17kD (p17) and 12kD (p12). Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins.
Format :
Purified
Formulation :
0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Gene ID #:
836
Immunogen:
The large subunit domain of human Caspase-3 was used as the immunogen for this monoclonal antibody.
Limitation:
This Caspase-3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G purified monoclonal antibody
Species Reactivity :
Human, Mouse, Rat