CD28 Antibody

NSJ Bioreagents
Product Code: NSJ-V2051
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2051-20UG20 ug£264.00
Quantity:
NSJ-V2051-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CB28
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Functional Study
  • Immunofluorescence (IF)
Storage:
Store the CD28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Flow cytometry staining of PFA-fixed human Jukat cells with CD28 antibody (clone CB28); Red=isotype control, Blue= CD28 antibody.
2 / 3
Immunofluorescent staining of PFA-fixed human Jurkat cells with CD28 antibody (clone CB28, green) and Reddot nuclear stain (red).
3 / 3
SDS-PAGE analysis of purified, BSA-free CD28 antibody (clone CB28) as confirmation of integrity and purity.

Flow cytometry staining of PFA-fixed human Jukat cells with CD28 antibody (clone CB28); Red=isotype control, Blue= CD28 antibody.
Immunofluorescent staining of PFA-fixed human Jurkat cells with CD28 antibody (clone CB28, green) and Reddot nuclear stain (red).
SDS-PAGE analysis of purified, BSA-free CD28 antibody (clone CB28) as confirmation of integrity and purity.

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Functional studies (order BSA/sodium azide-free format)
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD28 antibody to be titered up or down for optimal performance.
Description:
This antibody recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical Tcell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing Tcells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. it is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
940
Immunogen:
Recombinant human protein was used as the immunogen for this CD28 antibody.
Limitation:
This CD28 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human