CD40L Antibody / TRAP / CD154

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NSJ Bioreagents
Product Code: NSJ-V8248
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8248-20UG20 ug£264.00
Quantity:
NSJ-V8248-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CD40LG/2763
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the CD40L antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 6
Flow testing of human Jurkat cells with CD40L antibody (blue) and isotype control (red).
2 / 6
Immunofluorescent staining of human Jurkat cells with CD40L antibody (green) and Reddot nuclear stain (red).
3 / 6
IHC testing of FFPE human spleen with CD40L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 6
IHC testing of FFPE human spleen with CD40L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 6
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD40L antibody (clone CD40LG/2763). These results demonstrate the foremost specificity of the CD40LG/2763 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
6 / 6
SDS-PAGE analysis of purified, BSA-free CD40L antibody (clone CD40LG/2763) as confirmation of integrity and purity.
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Flow testing of human Jurkat cells with CD40L antibody (blue) and isotype control (red).
Immunofluorescent staining of human Jurkat cells with CD40L antibody (green) and Reddot nuclear stain (red).
IHC testing of FFPE human spleen with CD40L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human spleen with CD40L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD40L antibody (clone CD40LG/2763). These results demonstrate the foremost specificity of the CD40LG/2763 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CD40L antibody (clone CD40LG/2763) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 108-261 from the human protein was used as the immunogen for the CD40L antibody.
Limitation:
This CD40L antibody is available for research use only.
Localization:
Cell surface, secreted
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P29965