E-Cadherin Antibody / CDH1

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NSJ Bioreagents
Product Code: NSJ-V3565
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3565-20UG20 ug£264.00
Quantity:
NSJ-V3565-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: CDH1/2208R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone CDH1/2208R, green) and Reddot nuclear stain (red).
2 / 3
Formalin-fixed, paraffin-embedded human colon carcinoma stained with recombinant E-Cadherin antibody (clone CDH1/2208R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant E-Cadherin antibody (clone CDH1/2208R) as confirmation of integrity and purity.
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Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone CDH1/2208R, green) and Reddot nuclear stain (red).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with recombinant E-Cadherin antibody (clone CDH1/2208R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant E-Cadherin antibody (clone CDH1/2208R) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant E-Cadherin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Cadherins behave as both receptors and ligands for other molecules. During development, their behavior assists in properly positioning cells: they are responsible for the separation of the different tissue layers, and for cellular migration. In the very early stages of development, E-cadherin (epithelial cadherin) is most greatly expressed. The E-cadherin-catenin complex plays a key role in cellular adhesion; loss of this function has been associated with greater tumour metastasis. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant E-Cadherin antibody.
Limitation:
This recombinant E-Cadherin antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P12830