Cadherin 17 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7652
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7652-20UG20 ug£264.00
Quantity:
NSJ-V7652-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CDH17/2618
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Cadherin 17 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
Flow cytometry testing of human MCF7 cells with Cadherin 17 antibody (clone CDH17/2618); Red=isotype control, Blue= Cadherin 17 antibody.
2 / 4
IHC staining of FFPE human colon with Cadherin 17 antibody (clone CDH17/2618). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Cadherin 17 antibody (clone CDH17/2618). These results demonstrate the foremost specificity of the CDH17/2618 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free Cadherin 17 antibody (clone CDH17/2618) as confirmation of integrity and purity.
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Flow cytometry testing of human MCF7 cells with Cadherin 17 antibody (clone CDH17/2618); Red=isotype control, Blue= Cadherin 17 antibody.
IHC staining of FFPE human colon with Cadherin 17 antibody (clone CDH17/2618). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Cadherin 17 antibody (clone CDH17/2618). These results demonstrate the foremost specificity of the CDH17/2618 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Cadherin 17 antibody (clone CDH17/2618) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It recognizes a protein of 120kDa, which is identified as Cadherin 17 (also known as LI Cadherin). The cadherins are a family of Calcium-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. LI-cadherin (for liver-intestine-cadherin) expression is restricted to liver and intestine tissues and is specifically localized to the basolateral domain of hepatocytes and enterocytes.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amino acids 242-418) was used as the immunogen for the Cadherin 17 antibody.
Limitation:
This Cadherin 17 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q12864