N-Cadherin Antibody / CDH2

Immunohistochemistry (FFPE): 0.5-1ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cadherin 2 antibody to be titered up or down for optimal performance.

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as Cadherin 2, CDH2 and CD325. Cadherin 2 is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. Expression of Cadherin 2 has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. Results aid in the classification of malignant non-carcinomatous neoplasms including mesotheliomas, chordomas, synovial sarcomas, malignant melanomas, epithelioid sarcomas, epithelioid angiosarcomas, clear cell sarcomas as well as serous and endometrioid tumors of the ovary have been demonstrated to be Cadherin 2 positive, whereas mucinous tumors are negative. Other Cadherin 2-positive neoplasms include renal cell carcinomas and some variant breast tumors, including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

A human partial recombinant protein corresponding to the intracellular portion of CDH2 was used as the immunogen for this Cadherin 2 antibody.

This Cadherin 2 antibody is available for research use only.

Cell surface, cytoplasmic

Protein G affinity chromatography

Human, Mouse

P19022