CD14 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3764
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3764-20UG20 ug£264.00
Quantity:
NSJ-V3764-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CDLA14-2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CD14 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human lymph node with CD14 antibody (clone CDLA14-2). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.~
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Western blot testing of human A549 cell lysate wi
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IHC testing of FFPE human lymph node with CD14 antibody (clone CDLA14-2). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.~
Western blot testing of human A549 cell lysate wi

Documents

Further Information

Application Details :
IHC (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 25-148 was used as immunogen for this CD14 antibody.
Limitation:
This CD14 antibody is available for research use only.
Localization:
Cell surface, Secreted, Cytoplasmic (Golgi)
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P08571