CD22 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3786
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3786-20UG20 ug£264.00
Quantity:
NSJ-V3786-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CDLA22-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store the CD22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human Raji cell lysate with CD22 antibody (clone CDLA22-1). Expected molecular weight: 76-150 kDa depending on glycosylation level.~
2 / 2
Flow cytometry testing of human Ramos cells with CD22 antibody (clone CDLA22-1);
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Western blot testing of human Raji cell lysate with CD22 antibody (clone CDLA22-1). Expected molecular weight: 76-150 kDa depending on glycosylation level.~
Flow cytometry testing of human Ramos cells with CD22 antibody (clone CDLA22-1);

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the CD22 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. [Uniprot]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Amino acids 52-178 from the human protein were used as the immunogen for the CD22 antibody.
Limitation:
This CD22 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P20273