CD43 Antibody

NSJ Bioreagents
Product Code: NSJ-V7572
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7572-20UG20 ug£264.00
Quantity:
NSJ-V7572-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CDLA43-2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Immunofluorescence staining of human K562 cells with CD43 antibody (green, clone CDLA43-2) and NucSpot Live 650 (red).
2 / 3
IHC staining of FFPE human spleen with CD43 antibody (clone CDLA43-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 3
Western blot testing of human K562 cell lysate with CD43 antibody (clone CDLA43-2). Expected molecular weight: 45-135 kDa depending on glycosylation level.

Immunofluorescence staining of human K562 cells with CD43 antibody (green, clone CDLA43-2) and NucSpot Live 650 (red).
IHC staining of FFPE human spleen with CD43 antibody (clone CDLA43-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human K562 cell lysate with CD43 antibody (clone CDLA43-2). Expected molecular weight: 45-135 kDa depending on glycosylation level.

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the CD43 antibody should be determined by the researcher.
Description:
CD43 is one of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human SPN protein was used as the immunogen for the CD43 antibody.
Limitation:
This CD43 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P16150