CD73 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7559
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7559-20UG20 ug£264.00
Quantity:
NSJ-V7559-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CDLA73-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CD73 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Flow cytometry testing of human U-87 MG cells with CD73 antibody (clone CDLA73-1); Red=isotype control, Blue= CD73 antibody.
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Immunofluorescence staining of human U-87 MG cells with CD73 antibody (green, clone CDLA73-1) and Reddot nuclear stain (red).
3 / 3
Western blot testing of human 1) A431 and 2) PC-3 cell lysate with CD73 antibody (clone CDLA73-1). Predicted molecular weight: 60-70 kDa.
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Flow cytometry testing of human U-87 MG cells with CD73 antibody (clone CDLA73-1); Red=isotype control, Blue= CD73 antibody.
Immunofluorescence staining of human U-87 MG cells with CD73 antibody (green, clone CDLA73-1) and Reddot nuclear stain (red).
Western blot testing of human 1) A431 and 2) PC-3 cell lysate with CD73 antibody (clone CDLA73-1). Predicted molecular weight: 60-70 kDa.

Documents

Further Information

Application Details :
ELISA: order BSA-free format for coating,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the CD73 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
The protein encoded by the NT5E gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. [RefSeq]
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.
Limitation:
This CD73 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P21589