CD74 / CLIP Antibody

Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry: 1-2ug/ml,Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the CD74 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

CD74 associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [RefSeq]

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Recombinant full-length human protein was used as the immunogen for the CD74 antibody. The epitope is known to be extracellular.

This CD74 antibody is available for research use only.

Cell surface and paranuclear globular

Protein G affinity chromatography

Human

P04233