CELA3B Antibody / Elastase 3B

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NSJ Bioreagents
Product Code: NSJ-V3436
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3436-20UG20 ug£264.00
Quantity:
NSJ-V3436-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CELA3B/1257
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Elastase 3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
IHC testing of FFPE human pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2 / 3
IHC testing of FFPE mouse pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
Western blot of human PANC-1 cell lysate using Elastase 3B antibody (CELA3B/1257). Predicted molecular weight ~29 kDa.
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IHC testing of FFPE human pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE mouse pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
Western blot of human PANC-1 cell lysate using Elastase 3B antibody (CELA3B/1257). Predicted molecular weight ~29 kDa.

Documents

Further Information

Application Details :
ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

1. FFPE staining requires

Description:
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.
Limitation:
This Elastase 3B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P08861