p21 Antibody / CDKN1A

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NSJ Bioreagents
Product Code: NSJ-V8615
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8615-20UG20 ug£264.00
Quantity:
NSJ-V8615-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: CIP1/4377R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant p21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using p21 antibody. These results demonstrate the foremost specificity of the CIP1/4377R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
IHC staining of FFPE human lung carcinoma with recombinant p21 antibody (clone CIP1/4377R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
IHC staining of FFPE human colon carcinoma with recombinant p21 antibody (clone CIP1/4377R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using p21 antibody. These results demonstrate the foremost specificity of the CIP1/4377R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human lung carcinoma with recombinant p21 antibody (clone CIP1/4377R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with recombinant p21 antibody (clone CIP1/4377R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the recombinant p21 antibody should be determined by the researcher.
Description:
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 1-100 from the human protein was used as the immunogen for the recombinant p21 antibody.
Limitation:
This recombinant p21 antibody is available for research use only.
Localization:
Nuclear
Microarray Validated?:
Yes
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P38936