Calponin 1 Antibody

NSJ Bioreagents
Product Code: NSJ-V7050
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7050-20UG20 ug£264.00
Quantity:
NSJ-V7050-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CLPN5-1
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 2
Immunofluorescent staining of PFA-fixed human K562 cells with Calponin antibody (green, clone CLPN5-1) and Reddot nuclear stain (red).~
2 / 2
IHC testing of FFPE human uterus and Calponin antibody (clone CLPN5-1). Staining of formalin-fixed ti

Immunofluorescent staining of PFA-fixed human K562 cells with Calponin antibody (green, clone CLPN5-1) and Reddot nuclear stain (red).~
IHC testing of FFPE human uterus and Calponin antibody (clone CLPN5-1). Staining of formalin-fixed ti

Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the Calponin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the Calponin antibody.
Limitation:
This Calponin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Rat
Uniprot #:
P51911