Calponin 1 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7248
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7248-20UG20 ug£264.00
Quantity:
NSJ-V7248-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: CNN1/1408R
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
2 / 3
IHC testing of FFPE rat uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Calponin antibody (clone CNN1/1408R) as confirmation of integrity and purity.
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IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Calponin antibody (clone CNN1/1408R) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.
Limitation:
This recombinant Calponin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human, Rat
Uniprot #:
P51911