Cystatin B Antibody / CSTB / Stefin B

NSJ Bioreagents
Product Code: NSJ-V8489
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8489-20UG20 ug£264.00
Quantity:
NSJ-V8489-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CPTC-CSTB-3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cystatin B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human lung with Cystatin B antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2
SDS-PAGE analysis of purified, BSA-free Cystatin B antibody as confirmatio

IHC staining of FFPE human lung with Cystatin B antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
SDS-PAGE analysis of purified, BSA-free Cystatin B antibody as confirmatio

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Cystatin B antibody should be determined by the researcher.
Description:
Cystatin A (also designated STF1, STFA, stefin A or cystatin AS) and cystatin B (also designated PME, CST6, STFB, CPI-B, stefin B and liver thiol proteinase inhibitor) are thiol protease inhibitors that form complexes with papain and the cathepsins B, H, and L. cystatin A, a cytoplasmic protein, is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. cystatin B protects against intracellular proteases leaking out of lysosomes and is primarily expressed in heart, liver and kidney.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human Cystatin B protein was used as the immunogen for the Cystatin B antibody.
Limitation:
This Cystatin B antibody is available for research use only.
Localization:
Cytoplasm and nucleus
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P04080